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Electron transport chain Transitional Reasoning Practice

Biology · Cellular Energetics · Biology · Cellular Respiration

1 Biology practice questions on Electron transport chain. Questions that build towards applied reasoning.

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  1. 1.

    Researchers isolated functional mitochondria in an isotonic buffer containing pyruvate, ADP, and inorganic phosphate to monitor cellular energetics. Upon adding an experimental reagent, the rate of oxygen consumption increased markedly, yet ATP synthesis stopped completely and the proton gradient across the inner mitochondrial membrane collapsed. Based on these experimental findings, which statement best describes the mechanism of action of this reagent?

    • A) It binds irreversibly to Complex IV, preventing electron transfer to molecular oxygen.
    • B) It selectively blocks the catalytic site of ATP synthase, preventing ADP phosphorylation without affecting proton flow.
    • C) It halts pyruvate import into the matrix, depriving the citric acid cycle of substrate for NADH generation.
    • D) It increases the permeability of the inner mitochondrial membrane to protons, uncoupling electron transport from ATP synthesis.

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