In an in vitro gene expression assay, an investigator supplies a purified bacterial RNA polymerase with the four standard ribonucleoside triphosphates (ATP, CTP, GTP, and UTP) and a linear double-stranded DNA fragment. Transcription initiates at the promoter, reading the designated template strand with the sequence 3'-TACGGACTTGCA-5'. Which of the following correctly identifies the sequence of the synthesized RNA molecule along with a fundamental biochemical feature of transcription?

A) 5'-AUGCCUGAACGU-3', synthesized in the 3' to 5' direction using deoxyribonucleotide precursors.

B) 5'-UACGGACUUGCA-3', synthesized in the 5' to 3' direction with the mandatory assistance of an RNA primase.

C) 3'-AUGCCUGAACGU-5', synthesized antiparallel to the coding strand by utilizing pre-existing 3'-OH primers.

D) 5'-AUGCCUGAACGU-3', synthesized in the 5' to 3' direction by RNA polymerase without requiring an initial primer.

Transcription Transitional Reasoning Practice

Biology · Molecular Genetics · Biology · Gene Expression

1 Biology practice questions on Transcription. Questions that build towards applied reasoning.

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